I'm a Guitar player... and once, years ago, a music shop owner said to me that it's the FIRST time he has seen anybody
check a guitars 'tuning' with a 'comb' !!
What I was doing, (and often do!), is pluck strings while using the back edge
of my comb without fully depressing the strings, to create additional 'harmonics', so that I quickly and easily check for the
position directly over certain built-in Fret positions including the '12th'. As you say, the position of the 'Bridge' adds a whole
new dimension to 'Open-Tuning'. Often in regard to say Steel-String guitars, the position/shape/angle of the 'bridge' is
fixed, and greatly affects the 'intonation' of the instrument, when playing up the frets!
THIS is often quite subjective in results, as one also needs to consider the height of the strings above the Fret-Board,
(as varying people like/prefer), because one needs to consider that to 'depress' that string all the way to the FretBoard
results in that string being 'stretched further' slightly too, affecting the tonal qualities/accuracy as you move up the frets!!
The BEST we can do, is to settle on an 'average', depending on the chords/notes played, and in the KEY we are in.
The biggest upsetting of what I mentioned & why, is the use of a CAPO, and why/when.